A Study of Next Generation Sequencing Data, Workflow, Application and Platform Comparison


DNA sequencing determines the precise order of nucleotides within a DNA molecule.Next generation Sequencing (NGS) is an efficient parallel high throughput DNA Sequencing Technology which revolutionizing the genomic research. Earlier methods that are quite expensive give rise to different sequence comparison techniques. NGS used for faster detection of variants in human genome and give rise to accelerated response to disease detection like cancer, hepatitis etc. In this paper work flow of data analysis part of NGS are clearly discussed. The different NGS platforms, its applications and different sequencing comparison techniques are also mentioned here.

DNA Sequencing is a fundamental terminology in Bioinformatics. Genes are the fundamental element in a cell that makes up the organism .The process of determining the precise order of nucleotides Adenine(A) ,Guanine(G), Cytosine(C) ,Thymine(T) within a DNA molecule is referred to as DNA sequencing. The order of the bases along DNA contains the complete set of instructions that make up the genetic inheritance. The key principle of DNA sequence generation method is to evaluate the sequencing with very high accuracy and reliability.

Next generation sequencing (NGS) Technology which is a high through put parallel sequencing technology which accelerated the genomic research.NGS deals with enormous amount of data as the different combination of nucleotides which is then converted into a string problem in computer science.[12] Applications of NGS includes personalized medicine, Genetic Disorder, clinical diagnosis of different viral disease like cancer, hepatitis etc.It provide greater opportunities for fast and low cost sequencing in the modern times. Viral transmission like hepatitis, cancer, HIV can be efficiently detected by using NGS Technologies. International research project like The Human Genome Project (HGP) is a remarkable achievement of NGS. It is a program that aims to provide complete mapping of all set of genomes and will act as a world resource of detailed information about the function, structure and organization of the complete set of human genomes. After HGP the time, cost of comparison started decreasing while amount of scientific knowledge has continued growing exponentially. So NGS has its own significance to understand the code of life.
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Lucy Morgan
Editorial Coordinator
Journal of Next Generation Sequencing & Applications
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